Part:BBa_K4638021

EX-HA-MUT

Synonymous mutated Hemagglutinin (HA) of WSN in the signaling sequence of Neuraminidase (NA)

Usage and Biology

The neuraminidase (NA) of influenza A viruses, a class II glycoprotein with the amino terminus inside the cell and the carboxyl terminus outside is one of two major glycoproteins on the virus surface. It can help the virus to get into cells through endocytosis. The other glycoprotein is Hemagglutinin. Hemagglutinin, often abbreviated as HA, is a crucial protein found on the surface of influenza viruses. The HA protein binds to sialic acid-containing receptors on the host cell surface and mediates the fusion of the viral envelope with the endosomal membrane after receptor-mediated endocytosis. On the surface of Influenza, the expression of HA is often more than the expression of NA. We exchange the expressing signal sequence of these two proteins, hoping to enhance the virus’ ability to TfR1. This composite part is the HA in the signaling sequence of NA, using mutated signaling sequence and synonymously mutated HA protein.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1436
  Illegal SpeI site found at 1959
  Illegal PstI site found at 1877
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1436
  Illegal SpeI site found at 1959
  Illegal PstI site found at 1877
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1436
  Illegal BglII site found at 532
  Illegal XhoI site found at 311
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1436
  Illegal SpeI site found at 1959
  Illegal PstI site found at 1877
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1436
  Illegal SpeI site found at 1959
  Illegal PstI site found at 1877
  
• 1000
  COMPATIBLE WITH RFC[1000]